Concept: BDS_ER_CALUX in the ANALYS_MET vocabulary

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Concept URI	https://kodlistor.miljodatasamverkan.se/def/vocabulary/DATA_MATVARDE/ANALYS_MET/BDS_ER_CALUX
Preferred label	BDS_ER_CALUX
Definition	BDS-ER CALUX Analytical method from BioDetection Systems (BDS): Bioassay detection of estrogen signalling of ER-activity (agonistic/antagonistic) for 17β-estradiol. The samples were freeze dried, extracted with ASE (accelerated solvent extraction) with 100% acetone, restituted in DMSO (dimethylsulfoxid) and subsequently measured in the CALUX bioassay. ER CALUX bioassay comprise a human breast cancer cell line (T47D), incorporating the firefly luciferase gene coupled to Estrogen Responsive Elements (EREs) as a reporter gene. Following binding of estrogen compounds to the intracellular estrogen alpha and beta receptors, the ligand-receptor complexes bind to and activate the ERE followed by expression of estrogens and luciferace. By addition of substrate for luciferase, light is emitted which is measured in a luminometer. The amount of light produced is proportional to the amount of ligand-specific receptor binding, which is benchmarked against the reference compound: 17β-estradiol. The amount of light of the samples is interpolated in the curve to obtain the reference equivalents. (Van der Burg, B., Van der Linden, S.C., Man, H.Y., Winter, R., Jonker, L., Van Vugt-Lussenburg, B., Brouwer, A. (2013) A panel of quantitative CALUX® reporter gene assays for reliable high throughput toxicity screening of chemicals and complex mixtures. In “High throughput screening methods in toxicity testing” (P. Steinberg, ed). John Wiley and Sons, Inc. New York. ISBN 9781118065631 pp. 519-532)
Notation
Status	Valid
Status Modified	2025-07-08
Accepted Date	2025-07-08
Not Accepted Date